Hierarchical Clustering of medaka and Xiphophorus array features for brain, liver, and gill tissues

Data in the following pages are representative information from "Use of a medaka based microarray to assess hypoxia-induced changes in Xiphophorus maculatus gene expression " (CBP, Toxicology and Pharmacology). Medaka and Xiphophorus were co-exposed to either hypoxic or normoxic conditions for 120 hrs; both species were sacrificed at that time and individual tissues were dissected. RNA from each of the three tissues was extracted from both medaka and Xiphophorus and analyzed using the 8 K medaka array. Panels A and B are line heat map representations of array features which were found to be significant (p-value <0.05) in both Xiphophorus (top) and medaka (bottom) within liver and gill, respectively. Within these two panels, all fold changes are considered to view the global similarity between the two species in each of the respective tissues. Panel C is a block heat map representation of significantly (p-value <0.05 and minimum fold change of 2 in either direction) modulated genes derived from hybridization of the 8 K array with RNA derived from brain. This panel represents the conserved nature between the two species (Xiphophorus on top and medaka on bottom) for genes experiencing extreme fold changes. In each of the figures, green represents those features that indicated less abundance of the target RNA vs. control RNA (i.e. down regulated), while red represents greater abundant target sequences relative to control RNA (i.e., up regulated genes). Links to all individual array features used to develop the heat maps shown in panels A, B, and C may be accessed by clicking on the individual tissue maps above.