Stage 22
 

Age, 13.4 days.

Standard length, 4.00 mm. Length of caudal fin, 0.79 mm. Mesencephalon width, 1.05 mm. Depth of yolk, 1.19 mm.

The yolk mass is further reduced, and there is a concomitant regression of the extra-embryonic membranes to form a “neck strap,” covering the posterior one-third of the eye and extending posteriorly to cover most of the mesencephalic region. Fin rays in the anal fin make their first appearance here.